PIAS3 Colorimetric Cell-Based ELISA Kit
The PIAS3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PIAS3 protein expression profile in cells. The kit can be used for measuring the relative amounts of PIAS3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PIAS3.
Product Specifications
Synonyms
E3 SUMO-protein ligase PIAS3;2.3.2.-; E3 SUMO-protein transferase PIAS3 ; Protein inhibitor of activated STAT protein 3; PIAS3
Gene Name
PIAS3
UniProt
Q9Y6X2
Reactivity
Human, Mouse, Rat
Tissue Specificity
Widely expressed.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184) . Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032) . Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376) .
Molecular Weight
68017 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
E3 SUMO-protein ligase PIAS3
Subcellular Location
Cytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
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