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PHCA Colorimetric Cell-Based ELISA Kit

The PHCA Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PHCA protein expression profile in cells. The kit can be used for measuring the relative amounts of PHCA in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PHCA.

Product Specifications

Synonyms

Alkaline ceramidase 3; AlkCDase 3; Alkaline CDase 3;3.5.1.-; Alkaline dihydroceramidase SB89; Alkaline phytoceramidase; aPHC; ACER3; APHC, PHCA

Gene Name

ACER3

UniProt

Q9NUN7

Reactivity

Human, Mouse

Tissue Specificity

Ubiquitously expressed. Highest expression in placenta.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.

Molecular Weight

31552 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Alkaline ceramidase 3

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.

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