PDE4D Colorimetric Cell-Based ELISA Kit
The PDE4D Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PDE4D protein expression profile in cells. The kit can be used for measuring the relative amounts of PDE4D in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PDE4D.
Product Specifications
Synonyms
CAMP-specific 3',5'-cyclic phosphodiesterase 4D;3.1.4.53; DPDE3; PDE43; PDE4D; DPDE3
Gene Name
PDE4D
UniProt
Q08499
Reactivity
Human, Mouse, Rat
Tissue Specificity
Expressed in colonic epithelial cells (at protein level) . Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Molecular Weight
91115 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
CAMP-specific 3',5'-cyclic phosphodiesterase 4D
Subcellular Location
Apical cell membrane. Cytoplasm. Membrane. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity) . Colocalized with SHANK2 to the apical membrane of colonic crypt cells.
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