P57 Kip2 Colorimetric Cell-Based ELISA Kit

The p57 Kip2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p57 Kip2 protein expression profile in cells. The kit can be used for measuring the relative amounts of p57 Kip2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p57 Kip2.

Product Specifications

Synonyms

Cyclin-dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57; p57Kip2; CDKN1C; KIP2

Gene Name

CDKN1C

UniProt

P49918

Reactivity

Human, Mouse

Tissue Specificity

Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non- proliferative state throughout life.