P21 Cip1 Colorimetric Cell-Based ELISA Kit
The p21 Cip1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p21 Cip1 protein expression profile in cells. The kit can be used for measuring the relative amounts of p21 Cip1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p21 Cip1.
Product Specifications
Synonyms
Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21; CDKN1A; CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1
Gene Name
CDKN1A
UniProt
P38936
Reactivity
Human, Mouse, Rat
Tissue Specificity
Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739) .
Molecular Weight
18119 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Cyclin-dependent kinase inhibitor 1
Subcellular Location
Cytoplasm. Nucleus.
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