NM23 Colorimetric Cell-Based ELISA Kit
The NM23 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NM23 protein expression profile in cells. The kit can be used for measuring the relative amounts of NM23 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on NM23.
Product Specifications
Synonyms
Nucleoside diphosphate kinase B; NDK B; NDP kinase B;2.7.4.6 ; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB;2.7.13.3 ; nm23-H2; NME2; NM23B
Gene Name
NME2
UniProt
P22392
Reactivity
Human, Mouse, Rat
Tissue Specificity
Isoform 1 and isoform 3 are ubiquitously expressed.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity) . Negatively regulates Rho activity by interacting with AKAP13/LBC (PubMed:15249197) . Acts as a transcriptional activator of the MYC gene; binds DNA non- specifically (PubMed:8392752, PubMed:19435876) . Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III (1) region of the MYC gene promoter. Does not bind to duplex NHE III (1) (PubMed:19435876) . Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds both folded and unfolded G4 with similar low nanomolar affinities. Stabilizes folded G4s regardless of whether they are prefolded or not (PubMed:25679041) . Exhibits histidine protein kinase activity (PubMed:20946858) .
Molecular Weight
17298 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Product Datasheet
https://www.bosterbio.com/datasheet?sku=EKC1403
Product MSDS
https://www.bosterbio.com/msds?sku=EKC1403
Other Gene Names
Nucleoside diphosphate kinase B
Subcellular Location
Cytoplasm. Nucleus. Cell projection, lamellipodium. Cell projection, ruffle. Isoform 3 is mainly cytoplasmic and isoform 1 and isoform 3 are excluded from the nucleolus. Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates.
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