NEDD8 Colorimetric Cell-Based ELISA Kit

The NEDD8 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NEDD8 protein expression profile in cells. The kit can be used for measuring the relative amounts of NEDD8 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on NEDD8.

Product Specifications

Synonyms

NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8; NEDD8

Gene Name

NEDD8

UniProt

Q15843

Reactivity

Human, Mouse, Rat

Tissue Specificity

Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.