MART-1 Colorimetric Cell-Based ELISA Kit

The MART-1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MART-1 protein expression profile in cells. The kit can be used for measuring the relative amounts of MART-1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MART-1.

Product Specifications

Synonyms

Melanoma antigen recognized by T-cells 1; MART-1; Melan-A protein; Antigen SK29-AA; Antigen LB39-AA; MLANA; MART1

Gene Name

MLANA

UniProt

Q16655

Reactivity

Human

Tissue Specificity

Expression is restricted to melanoma and melanocyte cell lines and retina.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Molecular Weight

13157 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Melanoma antigen recognized by T-cells 1

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.