Integrin beta4 Colorimetric Cell-Based ELISA Kit

The Integrin beta4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Integrin beta4 protein expression profile in cells. The kit can be used for measuring the relative amounts of Integrin beta4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Integrin beta4.

Product Specifications

Synonyms

CD104 antigen; GP150; ITB4; integrin beta-4; integrin beta-4C

Gene Name

ITGB4

UniProt

P16144

Reactivity

Human, Mouse, Rat

Tissue Specificity

Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778) . ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760) .

Molecular Weight

202167 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Integrin beta-4

Subcellular Location

Cell membrane; Single-pass type I membrane protein.Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Colocalizes with DST at the leading edge of migrating keratinocytes.