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HSP40 Colorimetric Cell-Based ELISA Kit

The HSP40 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSP40 protein expression profile in cells. The kit can be used for measuring the relative amounts of HSP40 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSP40.

Product Specifications

Synonyms

DnaJ homolog subfamily B member 1; Heat shock 40 kDa protein 1; Heat shock protein 40; DnaJ protein homolog 1; HDJ-1; DNAJB1; DNAJ1; HDJ1; HSPF1

Gene Name

DNAJB1

UniProt

P25685

Reactivity

Human, Mouse, Rat

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401) . Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877) .

Molecular Weight

38044 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

DnaJ homolog subfamily B member 1

Subcellular Location

Cytoplasm. Nucleus. Nucleus, nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.

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