HSP27 Colorimetric Cell-Based ELISA Kit

The HSP27 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSP27 protein expression profile in cells. The kit can be used for measuring the relative amounts of HSP27 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSP27.

Product Specifications

Synonyms

28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 27 kDa protein; HSP 27; HSP25; HSPB1; P25; SRP27; Stress-responsive protein 27

Gene Name

HSPB1

UniProt

P04792

Reactivity

Human, Mouse, Rat

Tissue Specificity

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding- competent state (PubMed:10383393, PubMed:20178975) . Plays a role in stress resistance and actin organization (PubMed:19166925) . Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742) .

Molecular Weight

22783 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Heat shock protein beta-1

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub- nuclear structures known as SC35 speckles or nuclear splicing speckles.