HnRNP K Colorimetric Cell-Based ELISA Kit
The hnRNP K Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor hnRNP K protein expression profile in cells. The kit can be used for measuring the relative amounts of hnRNP K in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on hnRNP K.
Product Specifications
Synonyms
65 kDa phosphoprotein; CSBP; DC-stretch binding protein; Heterogeneous nuclear ribonucleoprotein K; hnRNP-K; HNRNPK; HNRPK; Transformation upregulated nuclear protein; TUNP
Gene Name
HNRNPK
UniProt
P61978
Reactivity
Human, Mouse, Rat
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly (C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly (C) single- stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity) . As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.
Molecular Weight
50976 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Heterogeneous nuclear ribonucleoprotein K
Subcellular Location
Cytoplasm. Nucleus, nucleoplasm. Cell projection, podosome. Recruited to p53/TP53- responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036) . In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702) .
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