Histone H2A.X Colorimetric Cell-Based ELISA Kit

The Histone H2A.X Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Histone H2A.X protein expression profile in cells. The kit can be used for measuring the relative amounts of Histone H2A.X in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Histone H2A.X.

Product Specifications

Synonyms

H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X

Gene Name

H2AFX

UniProt

P16104

Reactivity

Human, Mouse, Rat

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.