HDAC4 Colorimetric Cell-Based ELISA Kit
The HDAC4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HDAC4 protein expression profile in cells. The kit can be used for measuring the relative amounts of HDAC4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HDAC4.
Product Specifications
Synonyms
HD4; Histone deacetylase 4; KIAA0288
Gene Name
HDAC4
UniProt
P56524
Reactivity
Human, Mouse, Rat
Tissue Specificity
Ubiquitous.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) . Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed:27708256) .
Molecular Weight
119040 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Product Datasheet
https://www.bosterbio.com/datasheet?sku=EKC1264
Product MSDS
https://www.bosterbio.com/msds?sku=EKC1264
Other Gene Names
Histone deacetylase 4
Subcellular Location
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation.
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