FHIT Colorimetric Cell-Based ELISA Kit
The FHIT Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FHIT protein expression profile in cells. The kit can be used for measuring the relative amounts of FHIT in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FHIT.
Product Specifications
Synonyms
AP3A hydrolase; AP3AASE; Bis (5'-adenosyl) -triphosphatase; Bis (5'-adenosyl) -triphosphatease; Dinucleosidetriphosphatase; Fragile histidine triad protein
Gene Name
FHIT
UniProt
P49789
Reactivity
Human
Tissue Specificity
Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Cleaves P (1) -P (3) -bis (5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P (1) -P (4) -bis (5'- adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P (1) -P (3) -bis (5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low- affinity Ca (2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.
Molecular Weight
16858 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Bis (5'-adenosyl) -triphosphatase
Subcellular Location
Cytoplasm. Mitochondrion. Nucleus.
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