EPHA2/3/4 Colorimetric Cell-Based ELISA Kit
The EPHA2/3/4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor EPHA2/3/4 protein expression profile in cells. The kit can be used for measuring the relative amounts of EPHA2/3/4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on EPHA2/3/4.
Product Specifications
Synonyms
ECK; Ephrin type-A receptor 2 precursor; Epithelial cell kinase; MPK-5; SEK-2; SEK2; Tyrosine-protein kinase receptor ECK; kinase EphA2
Gene Name
EPHA2
UniProt
P29317
Reactivity
Human, Rat
Tissue Specificity
Expressed in brain and glioma tissue and glioma cell lines (at protein level) . Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand- independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
Molecular Weight
108266 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Ephrin type-A receptor 2
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell junction, focal adhesion. Present at regions of cell- cell contacts but also at the leading edge of migrating cells (PubMed:19573808, PubMed:20861311) . Relocates from the plasma membrane to the cytoplasmic and perinuclear regions in cancer cells (PubMed:18794797) .
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