E2F1 Colorimetric Cell-Based ELISA Kit
The E2F1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor E2F1 protein expression profile in cells. The kit can be used for measuring the relative amounts of E2F1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on E2F1.
Product Specifications
Synonyms
E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; RBBP3; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Transcription factor E2F1
Gene Name
E2F1
UniProt
Q01094
Reactivity
Human, Mouse
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812) . Positively regulates transcription of RRP1B (PubMed:20040599) .
Molecular Weight
46920 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Transcription factor E2F1
Subcellular Location
Nucleus.
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