E2F1 Colorimetric Cell-Based ELISA Kit

The E2F1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor E2F1 protein expression profile in cells. The kit can be used for measuring the relative amounts of E2F1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on E2F1.

Product Specifications

Synonyms

E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; RBBP3; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Transcription factor E2F1

Gene Name

E2F1

UniProt

Q01094

Reactivity

Human, Mouse

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812) . Positively regulates transcription of RRP1B (PubMed:20040599) .