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DP-1 Colorimetric Cell-Based ELISA Kit

The DP-1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor DP-1 protein expression profile in cells. The kit can be used for measuring the relative amounts of DP-1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DP-1.

Product Specifications

Synonyms

Transcription factor Dp-1; DRTF1-polypeptide 1; DRTF1; E2F dimerization partner 1; TFDP1; DP1

Gene Name

TFDP1

UniProt

Q14186

Reactivity

Human

Tissue Specificity

Highest levels in muscle. Also expressed in brain, placenta, liver and kidney. Lower levels in lung and pancreas. Not detected in heart.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed:8405995, PubMed:7739537) . The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed:20176812) .

Molecular Weight

45070 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Transcription factor Dp-1

Subcellular Location

Nucleus. Cytoplasm. Shuttles between the cytoplasm and nucleus and translocates into the nuclear compartment upon heterodimerization with E2F1.

Frequently Asked Questions

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