Claudin 1 Colorimetric Cell-Based ELISA Kit

The Claudin 1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Claudin 1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Claudin 1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Claudin 1.

Product Specifications

Synonyms

CLD1; CLDN1; SEMP1; Senescence-associated epithelial membrane protein

Gene Name

CLDN1

UniProt

O95832

Reactivity

Human, Mouse, Rat

Tissue Specificity

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391) .

Molecular Weight

22744 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Claudin-1

Subcellular Location

Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Basolateral cell membrane. Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.