Caspase 3 Colorimetric Cell-Based ELISA Kit
The Caspase 3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Caspase 3 protein expression profile in cells. The kit can be used for measuring the relative amounts of Caspase 3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Caspase 3.
Product Specifications
Synonyms
Apopain precursor; CASP-3; CPP-32; CPP32; Caspase-3; Cysteine protease CPP32; ICE3; SCA-1; SREBP cleavage activity 1; Yama protein
Gene Name
CASP3
UniProt
P42574
Reactivity
Human, Mouse, Rat
Tissue Specificity
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Molecular Weight
31608 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Caspase-3
Subcellular Location
Cytoplasm.
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