BID Colorimetric Cell-Based ELISA Kit

The BID Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor BID protein expression profile in cells. The kit can be used for measuring the relative amounts of BID in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on BID.

Product Specifications

Synonyms

BH3 interacting domain death agonist

Gene Name

BID

UniProt

P55957

Reactivity

Human, Mouse

Tissue Specificity

Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level) . Isoform 3 is expressed in lung, pancreas and spleen (at protein level) . Isoform 4 is expressed in lung and pancreas (at protein level) .

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

The major proteolytic product p15 BID allows the release of cytochrome c (By similarity) . Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.