BCL-2 Colorimetric Cell-Based ELISA Kit

The BCL-2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor BCL-2 protein expression profile in cells. The kit can be used for measuring the relative amounts of BCL-2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on BCL-2.

Product Specifications

Synonyms

Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; BCL2

Gene Name

BCL2

UniProt

P10415

Reactivity

Human, Mouse, Rat

Tissue Specificity

Expressed in a variety of tissues.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) . May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785) .

Molecular Weight

26266 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Apoptosis regulator Bcl-2

Subcellular Location

Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.