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AhR Colorimetric Cell-Based ELISA Kit

The AhR Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AhR protein expression profile in cells. The kit can be used for measuring the relative amounts of AhR in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AhR.

Product Specifications

Synonyms

Aryl hydrocarbon receptor; Ah receptor; AhR; Class E basic helix-loop-helix protein 76; bHLHe76; BHLHE76

Gene Name

AHR

UniProt

P35869

Reactivity

Human, Mouse, Rat

Tissue Specificity

Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) . Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.

Molecular Weight

96147 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Other Gene Names

Aryl hydrocarbon receptor

Subcellular Location

Cytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.

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