OCT1 Colorimetric Cell-Based ELISA Kit

The OCT1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor OCT1 protein expression profile in cells. The kit can be used for measuring the relative amounts of OCT1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on OCT1.

Product Specifications

Synonyms

NF-A1; OCT1; OTF-1; Octamer-binding transcription factor 1; PO21; PO2F1; POU domain, class 2; transcription factor 1; POU2F1

Gene Name

POU2F1

UniProt

P14859

Reactivity

Human, Mouse

Tissue Specificity

Ubiquitous. Isoform 2 is lymphocyte-specific.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity) . In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.