Anti-Phospho Histone H3.3 (Ser31) H3F3A Antibody

Boster Bio Anti-Phospho Histone H3.3 (Ser31) H3F3A Antibody (Catalog# P30442) . Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

Gene Name

H3F3A

UniProt

P84243

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A peptide derived from human Histone H3.3 around the phosphorylation site of Ser31. Immunogen sequence location: 16-65

Clonality

Polyclonal

Applications

IHC-P, IF, ICC, ELISA

Field of Research

2339, Epigenetics and Nuclear Signaling, Histones

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.