Anti-Phospho-p70 S6K (Thr398) Antibody

P70 S6 kinase (p70 S6K) is activated in a signaling pathway that includes mTOR and is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (Xio et al., 2009) . p70 S6K is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains and subsequently phosphorylates specifically ribosomal protein S6 (Saitoh et al., 2002) . Phosphorylation of Thr-229 in the catalytic domain and Thr-389 in the linker domain are most critical for kinase function. Inhibition of p70 activity inhibits the entry into S phase of the cell cycle and exhibits cell cycle arrest at G0/G1 phase, suggesting that the activation of p70 S6k plays an obligatory role in mediating mitogenic signals during cell activation (Xio et al., 2009) .

70 kDa ribosomal protein S6 kinase 2 antibody, EC 2.7.11.1 antibody, KS6B2_HUMAN antibody, p70 beta antibody, p70 ribosomal S6 kinase beta antibody, p70 S6 kinase beta antibody, p70 S6K-beta antibody, p70 S6KB antibody, p70 S6Kbeta antibody, p70 (S6K) beta antibody, p70-beta antibody, p70-S6K 2 antibody, P70S6K2 antibody, p70S6Kb antibody, Ribosomal protein S6 kinase 70kDa polypeptide 2 antibody, Ribosomal protein S6 kinase B2 antibody, Ribosomal protein S6 kinase beta 2 antibody, Ribosomal protein S6 kinase beta-2 antibody, Rps6kb2 antibody, S6 kinase related kinase antibody, S6 kinase-related kinase antibody, S6K beta 2 antibody, S6K beta antibody, S6K-beta antibody, S6K-beta-2 antibody, S6K2 antibody, Serine/threonine protein kinase 14 beta antibody, Serine/threonine-protein kinase 14B antibody, SRK antibody, STK14B antibody

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr398 of Drosophila p70 S6K protein, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Drosophila.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Specific for endogenous levels of the ~70 kDa p70 S6K protein phosphorylated at Thr398. Immunolabeling is blocked by preadsorption with the Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.