Anti-Phospho-DENND3 (Ser554) Antibody

The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (Marat, A.L., et al., 2011) . Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (Xu and McPherson, 2015; Matsui et al., 2011) . ULK-mediated phosphorylation of DENND3 at serines 554 and 572 upregulates its GEF activity toward the small GTPase Rab12 (Xu and McPherson, 2015) .

DENN Domain Containing 3, DENN Domain-Containing Protein 3, DENN/MADD Domain Containing 3, KIAA0870 DENN/MADD domain containing 3, KIAA0870DENN domain-containing protein 3

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser554 of human DENND3, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Human.

Guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form. Thereby, may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Ser554. The immunolabeling is completely eliminated by treatment with λ-phosphatase.