Anti-Selenophosphate Synthetase 1 SEPHS1 Antibody

Boster Bio Anti-Selenophosphate Synthetase 1 SEPHS1 Antibody (Catalog # P10102) . Tested in ELISA, WB applications. This antibody reacts with Mouse.

Product Specifications

Background

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Selenophosphate synthetase 1 (SPS1) (also known as selinide water dikinase or selenium donor protein 1) is the homolog of the SelD gene product in E. coli. In E. coli, SelD generates the selenium donor for selenocysteine tRNA biosynthesis. The function of SPS1 in mammals is still unknown but appears to differ from that of E. coli SelD.

Synonyms

Selenophosphate Synthetase 1, SPS1, Selenide water dikinase 1, Selenophosphate synthetase 1, Selenium donor protein 1

Gene Name

SEPHS1

UniProt

Q8BH69

Host

Rabbit

Reactivity

Mouse

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS1.

Clonality

Polyclonal

Tissue Specificity

Isoform 1 and isoform 2 are gradually expressed during the cell cycle until G2/M phase and then decreased. Isoform 3 is gradually expressed during the cell cycle until S phase and then decreased.

Applications

ELISA, WB

Field of Research

Cell Biology

Purification

This antibody is directed against mouse SPS1. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with SPS1 from mouse, human and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Concentration

85 mg/mL by Refractometry

Form

Lyophilized

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Function

Synthesizes selenophosphate from selenide and ATP.

Molecular Weight

42911 MW

Shipping Conditions

Available

Storage Conditions

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Fragment

Antiserum

Applications Notes

This antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to SPS1 by western blotting in the appropriate cell lysate or extract.