Anti-Phospho-Protein Tyrosine Phosphatase H1 (PTPH1) (Ser459) Antibody

Boster Bio Anti-Phospho-Protein Tyrosine Phosphatase H1 (PTPH1) (Ser459) Antibody (Catalog # P06227) . Tested in WB applications. This antibody reacts with Human, Mouse.

Product Specifications

Background

Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010) . Ras has been demonstrated to increase both p38γ and PTPH1 protein expression, and there is a coupling of increased p38γ and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010) . Phosphorylation of PTPH1 at Ser-459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012) . Additionally, phosphorylation of PTPH1 Ser-459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012) .

Phospho Site

Phosphorylated, Ser459

Synonyms

Cytoskeletal-associated protein tyrosine phosphatase antibody, protein tyrosine phosphatase H1 antibody, protein tyrosine phosphatase non-receptor type 3 antibody, Protein-tyrosine phosphatase H1 antibody, PTN3_HUMAN antibody, PTP-H1 antibody, PTPH1 antibody, PTPN3 antibody, Tyrosine-protein phosphatase non-receptor type 3 antibody

Gene Name

PTPH1

UniProt

P26045

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser459 of human PTPH1, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Human.

Clonality

Polyclonal

Applications

Field of Research

Protein Phosphorylation, Signal Transduction, Tyrosine Phosphatases

Purification

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

WB: 1:1000

Form

Liquid

Function

May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity.

Molecular Weight

103990 MW

Shipping Conditions

Available

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Fragment

IgG

Applications Notes

Specific for endogenous levels of the ~104 kDa PTPH1 protein phosphorylated at Ser459. The immunolabeling is completely eliminated by treatment with λ-phosphatase.