Anti-Phospho-Munc-18 (Ser513) STXBP2 Antibody

Boster Bio Anti-Phospho-Munc-18 (Ser513) STXBP2 Antibody (Catalog # P04909) . Tested in WB, ICC applications. This antibody reacts with Rat.

Product Specifications

Background

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005) . Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004) . The function of Unc-18 is thought to be regulated by PKC phosphorylation of Ser-515 in C. Elegans (Sassa et al., 1996) . Ser-513 is the mammalian homologue of Ser-515 in C. Elegans.

Phospho Site

Phosphorylated, Ser513

Synonyms

FLJ37475 antibody, Munc 18 1 antibody, Munc 18a antibody, MUNC18 1 antibody, N-Sec1 antibody, Neuronal SEC1 antibody, NSec1 antibody, p67 antibody, Protein unc-18 homolog 1 antibody, Protein unc-18 homolog A antibody, Rb sec1 antibody, RBSEC1 antibody, STXB1_HUMAN antibody, STXBP1 antibody, Syntaxin binding protein 1 antibody, Syntaxin-binding protein 1 antibody, Unc 18 homolog antibody, Unc 18A antibody, Unc-18A antibody, Unc18 1 antibody, UNC18 antibody, Unc18-1 antibody

Gene Name

STXBP2

UniProt

Q62753

Host

Rabbit

Reactivity

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser513 of rat Munc-18, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Rat.

Clonality

Polyclonal

Tissue Specificity

Placenta, lung, liver, kidney and pancreas, as well as in peripheral blood lymphocytes.

Applications

WB, ICC

Field of Research

Protein Trafficking, Signal Transduction, Vesicle Transport

Purification

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Involved in intracellular vesicle trafficking and vesicle fusion with membranes. Contributes to the granule exocytosis machinery through interaction with soluble N- ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that regulate membrane fusion. Regulates cytotoxic granule exocytosis in natural killer (NK) cells.

Molecular Weight

66453 MW

Shipping Conditions

Available

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Fragment

IgG

Applications Notes

Specific for endogenous levels of the ~65 kDa Munc-18 protein phosphorylated at Ser513. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.