Anti-Phospho-MEK5 (Ser311 Thr315) Antibody

Boster Bio Anti-Phospho-MEK5 (Ser311 Thr315) Antibody (Catalog # P03980) . Tested in WB applications. This antibody reacts with Rat.

Product Specifications

Background

MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999) . An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003) . Dual phosphorylation of Ser-311 and Thr-315 have been implicated in cell proliferation (Cameron et al., 2004) .

Phospho Site

Phosphorylated, Ser311, Thr315

Synonyms

Dual specificity mitogen activated protein kinase kinase 5 antibody, Dual specificity mitogen-activated protein kinase kinase 5 antibody, EC 2.7.12.2 antibody, HsT17454 antibody, MAP kinase kinase 5 antibody, MAP kinase kinase MEK5b antibody, MAP2K5 antibody, MAPK/ERK kinase 5 antibody, MAPKK 5 antibody, MAPKK5 antibody, MEK 5 antibody, mitogen-activated protein kinase kinase 5 antibody, MKK5 antibody, MP2K5_HUMAN antibody, PRKMK5 antibody, Protein kinase mitogen-activated kinase 5 antibody, SAPKK5 antibody, SKK5 antibody

Gene Name

MAP2K5

UniProt

Q62862

Host

Rabbit

Reactivity

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 of rat MEK5, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Rat.

Clonality

Polyclonal

Tissue Specificity

MEK5 beta is ubiquitously distributed with highest levels in the liver, whereas MEK5 alpha is expressed only in liver and brain.

Applications

Field of Research

Cancer, Cardiogenesis, Cardiovascular, MAPK Pathway, Protein Phosphorylation, Ser/Thr Kinases, Serine/Threonine Kinases, Signal Transduction, Transcription Factors/Regulators

Purification

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

WB: 1:1000

Form

Liquid

Function

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis (By similarity) .

Molecular Weight

50198 MW

Shipping Conditions

Available

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Fragment

IgG

Applications Notes

Specific for endogenous levels of the ~49 kDa MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.