Anti-Phospho-Che-1 (AATF) (Ser477) Antibody

Boster Bio Anti-Phospho-Che-1 (AATF) (Ser477) Antibody (Catalog # P03945) . Tested in WB applications. This antibody reacts with Human.

Product Specifications

Background

Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004) . It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004) . The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser-477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006) .

Phospho Site

Phosphorylated, Ser477

Synonyms

AATF antibody, AATF_HUMAN antibody, Apoptosis antagonizing transcription factor antibody, Apoptosis-antagonizing transcription factor antibody, BFR2 antibody, CHE 1 antibody, CHE1 antibody, DED antibody, Protein AATF antibody, Rb binding protein Che 1 antibody, Rb-binding protein Che-1 antibody

Gene Name

AATF

UniProt

Q9NY61

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 of human Che-1, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Human.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.

Applications

Field of Research

Apoptosis, Cell Biology, Epigenetics and Nuclear Signaling, Intracellular, Transcription

Purification

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

WB: 1:1000

Form

Liquid

Function

May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.

Molecular Weight

63133 MW

Shipping Conditions

Available

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Fragment

IgG

Applications Notes

Specific for endogenous levels of the ~66 kDa Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.