Anti-Phospho-ATRIP (Ser239) Antibody

Boster Bio Anti-Phospho-ATRIP (Ser239) Antibody (Catalog # P03862) . Tested in WB applications. This antibody reacts with Mouse.

Product Specifications

Background

ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001) . ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007) . DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007) . The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at Ser-239 (Venere et al, 2007) .

Phospho Site

Phosphorylated, Ser239

Synonyms

AGS1 antibody, ATIP antibody, ATM and Rad3 related interacting protein antibody, ATM and Rad3-related-interacting protein antibody, ATR interacting protein antibody, ATR-interacting protein antibody, Atrip antibody, ATRIP_HUMAN antibody, DKFZp762J2115 antibody, FLJ12343 antibody, MGC20625 antibody, MGC21482 antibody, MGC26740 antibody

Gene Name

ATRIP

UniProt

Q8WXE1

Host

Rabbit

Reactivity

Mouse

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 of human ATRIP, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Human.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

Field of Research

DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling

Purification

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

WB: 1:500

Form

Liquid

Function

Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.

Molecular Weight

85838 MW

Shipping Conditions

Available

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.