Anti-Phospho-eEF1A2 (Ser358) Antibody

Boster Bio Anti-Phospho-eEF1A2 (Ser358) Antibody (Catalog # P03304) . Tested in WB applications. This antibody reacts with Human, Mouse.

Product Specifications

Background

Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993) . Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002) . Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009) . Ribosome-associated JNK phosphorylates Ser-358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013) .

Phospho Site

Phosphorylated, Ser358

Synonyms

EEF1A-2 antibody, Eef1a2 antibody, EEF1AL antibody, EF 1 alpha 2 antibody, EF-1-alpha-2 antibody, EF1A antibody, EF1A2_HUMAN antibody, Elongation factor 1 A 2 antibody, Elongation factor 1 alpha antibody, Elongation factor 1 alpha 2 antibody, Elongation factor 1-alpha 2 antibody, Eukaryotic elongation factor 1 A-2 antibody, Eukaryotic translation elongation factor 1 alpha 2 antibody, FLJ41696 antibody, HS1 antibody, OTTHUMP00000031776 antibody, Statin like antibody, Statin S1 antibody, Statin-S1 antibody, STN antibody, STNL antibody

Gene Name

EEF1A2

UniProt

Q05639

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Human.

Clonality

Polyclonal

Tissue Specificity

Brain, heart, and skeletal muscle.

Applications

Field of Research

Cancer, DNA/RNA, Epigenetics and Nuclear Signaling, Oncoproteins, Oncoproteins/Suppressors, Ribosome, Transcription Factors, Translation

Purification

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

WB: 1:1000

Form

Liquid

Function

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Molecular Weight

50470 MW

Shipping Conditions

Available

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Fragment

IgG

Applications Notes

Specific for endogenous levels of the ~50 kDa eEF1A2 phosphorylated at Ser358. Immunolabeling is completely eliminated by treatment with λ-phosphatase.