Anti-Phospho-Synaptotagmin (Ser309) Syt1 Antibody

Boster Bio Anti-Phospho-Synaptotagmin (Ser309) Syt1 Antibody (Catalog # P02314-1) . Tested in WB, ICC applications. This antibody reacts with Rat.

Product Specifications

Background

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003) . Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003) . Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004) .

Phospho Site

Phosphorylated, Ser309

Synonyms

DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody

Gene Name

SYT1

UniProt

P21707

Host

Rabbit

Reactivity

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Rat.

Clonality

Polyclonal

Tissue Specificity

Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues.

Applications

WB, ICC

Field of Research

Neuroscience, Neurotransmission, Protein Trafficking, Secretory Vesicles, Signal Transduction, Vesicle Transport

Purification

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca (2+) -dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca (2+) -independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes.

Molecular Weight

47399 MW

Shipping Conditions

Available

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Fragment

IgG

Applications Notes

Specific for endogenous levels of the ~62 kDa synaptotagmin protein phosphorylated at Ser 309. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Other Gene Names

Synaptotagmin-1

Subcellular Location

Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass membrane protein. Cytoplasm. Synaptic vesicles and chromaffin granules.