Anti-Phospho-EphrinB (Tyr298) Antibody

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002) . EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cellto cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002) . Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr-298) plays a key role in EphrinB signaling (Kalo, et al., 2001) .

CEK5 ligand antibody, EFNB1 antibody, ELK L antibody, ELK ligand antibody, ELKL antibody, EPH related receptor tyrosine kinase ligand 2 antibody, Ephrin B1 antibody, Ephrin B2 antibody, LERK2 antibody

Synthetic phosphopeptide corresponding to amino acid residues surrounding the Tyr298 of Xenopus EphrinB, conjugated to keyhole limpet hemocyanin (KLH) . Note: Xenopus Tyr298 is the homolog of human, mouse and rat Tyr317 and also chicken Tyr305. Immunogen species is Xenopus.

Angiogenesis, Axonal Guidance Proteins, Cancer, Cardiovascular, Cell Type Marker, Growth and Development, Intracellular Signaling, Kinases, Neurology Process, Neuron Marker, Neuroscience, Neurotransmission, Protein Phosphorylation, Receptor Tyrosine Kinases, Receptors / Channels, Signal Transduction, Tyrosine Kinases

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Specific for endogenous levels of the ~46 kDa EphrinB protein phosphorylated at Tyr298. Immunolabeling is completely eliminated by treatment with λ-phosphatase.