Anti-Phospho-5-Lipoxygenase (Ser523) ALOX5 Antibody

The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001) . Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004) . Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004) . Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.

5 Lipoxygenase antibody, 5 LO antibody, 5 LOX antibody, 5-lipoxygenase antibody, 5-LO antibody, 5-LOX antibody, 5LOX antibody, 5LPG antibody, ALOX 5 antibody, Alox5 antibody, Arachidonate 5 lipoxygenase antibody, Arachidonate 5-lipoxygenase antibody, arachidonic 5-lipoxygenase alpha-10 isoform antibody, arachidonic 5-lipoxygenase delta-10-13 isoform antibody, arachidonic 5-lipoxygenase delta-13 isoform antibody, arachidonic 5-lipoxygenase delta-p10 isoform antibody, Arachidonic acid 5 lipoxygenase antibody, Leukotriene A4 synthase antibody, LOG 5 antibody, LOG5 antibody, LOX5_HUMAN antibody, MGC163204 antibody

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser523 of human 5-lipoxygenase (5-LO), conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Human.

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Endocrine Metabolism, Energy Metabolism, Energy Transfer Pathways, Heart Disease, Hormone Biosynthesis, Lipid and Lipoprotein Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Specific for endogenous levels of the ~80 kD 5-LO phosphorylated at Ser523 in Western blots. Immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-phosphatase, 1200 units for 30 min) .

Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.