Anti-Phospho-Connexin43 (Ser368) GJA1 Antibody

Boster Bio Anti-Phospho-Connexin43 (Ser368) GJA1 Antibody (Catalog # P00599) . Tested in WB applications. This antibody reacts with Mouse, Rat.

Product Specifications

Background

Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001) . Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser-368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a) . Phosphorylation of Ser-368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b) .

Phospho Site

Phosphorylated, Ser368

Synonyms

Connexin 43 antibody, Connexin-43 antibody, Cx 43 antibody, Cx43 antibody, CXA1_HUMAN antibody, DFNB38 antibody, Gap junction 43 kDa heart protein antibody, Gap junction alpha-1 protein antibody, Gap junction protein alpha 1 43kDa (connexin 43) antibody, Gap junction protein alpha 1 43kDa antibody, Gap junction protein alpha like antibody, GJA 1 antibody, Gja1 antibody, GJAL antibody, ODD antibody, ODDD antibody, ODOD antibody, SDTY3 antibody

Gene Name

GJA1

UniProt

P08050

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Rat.

Clonality

Polyclonal

Tissue Specificity

Expressed in the heart and fetal cochlea.

Applications

Field of Research

Atherosclerosis, Cardiovascular, Cell Adhesion, Cytoskeleton/ECM, Signal Transduction, Vascular Inflammation

Purification

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

WB: 1:1000

Form

Liquid

Function

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity) .

Molecular Weight

43008 MW

Shipping Conditions

Available

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Fragment

IgG

Applications Notes

Specific for endogenous levels of the ~43 kDa connexin-43 protein phosphorylated at Ser368. Immunolabeling is completely eliminated by treatment with λ-phosphatase.