Anti-Phospho-Progesterone Receptor (Ser294) PGR Antibody

There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005) . The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms) . In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003) . Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) . Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser-190 and Ser-294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.

NR3C3 antibody, Nuclear receptor subfamily 3 group C member 3 antibody, PGR antibody, PR antibody, PRA antibody, PRB antibody, PRGR_HUMAN antibody, Progesterone receptor antibody, Progestin receptor form A antibody, Progestin receptor form B antibody

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser294 of human progesterone receptor, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Human.

Cancer, Domain Families, Endocrine System, Epigenetics and Nuclear Signaling, Neuroscience, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Transcription, Tumor Biomarkers, Zinc Finger

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Specific for endogenous levels of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform phosphorylated at Ser294. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G (1) and G (2) /M phases.