Anti-Phospho-Serotonin Transporter (Thr276) SLC6A4 Antibody

The serotonin transporter (SERT) recycles serotonin by transporting it back to the pre-synaptic cell. It is the primary target for most anti-depressant drugs and for stimulants such as methamphetamines. SERT is regulated by several processes, including a cyclic GMP signaling pathway involving nitric oxide synthase, guanylyl cyclase, and cGMP-dependent protein kinase (PKG) . cGMP- and PKG-mediated SERT regulation requires phosphorylation at Thr-276 (Ramamoorthy et al., 2007) . It has been suggested that although PKG is involved in the stimulation of SERT at Thr-276, it does not directly phosphorylate the residue, rather it initiates a kinase cascade that leads to SERT phosphorylation by an as yet unidentified protein kinase (Wong et al., 2012) . Also of therapeutic importance, mutation at the Thr-276 residue has been shown to decrease the potency of a variety of anti-depressant drugs, (Zhang YW and Rudnick G, 2005) .

5 HTT antibody, 5 HTTLPR antibody, 5 hydroxytryptamine (serotonin) transporter antibody, 5 hydroxytryptamine transporter antibody, 5HT transporter antibody, 5HTT antibody, hSERT antibody, HTT antibody, Na+/Cl- dependent serotonin transporter antibody, OCD1 antibody, SC6A4_HUMAN antibody, Serotonin transporter 1 antibody, SERT antibody, SERT1 antibody, Slc6a4 antibody, Sodium dependent serotonin transporter antibody, Sodium-dependent serotonin transporter antibody, Solute carrier family 6 (neurotransmitter transporter) member 4 antibody, solute carrier family 6 (neurotransmitter transporter serotonin) member 4 antibody, Solute carrier family 6 member 4 antibody

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr276 of rat SERT, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Rat.

Drug Metabolism, Metabolic Signaling Pathways, Metabolism, Neuroscience, Neurotransmission, Neurotransmitter, Pathways and Processes, Receptors / Channels, Signal Transduction, Transporters

Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Specific for endogenous levels of the ~76 kDa SERT protein phosphorylated at Thr276. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin.