Anti-Phospho-Beclin-1 (Ser295) BECN1 Antibody

Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999) . Akt-mediated phosphorylation of beclin-1 at Ser-295 has been shown to decrease autophagy and promote oncogenesis (Wang et al., 2012) .

PG6 antibody, ATG 6 antibody, ATG6 antibody, ATG6 autophagy related 6 homolog antibody, Bcl-2-interacting protein beclin antibody, Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody, Beclin 1 autophagy related antibody, Beclin-1 antibody, Beclin1 antibody, BECN 1 antibody, Becn1 antibody, BECN1_HUMAN antibody, Coiled coil myosin like BCL2 interacting protein antibody, Coiled-coil myosin-like BCL2-interacting protein antibody, GT197 antibody, Protein GT197 antibody, VPS 30 antibody, VPS30 antibody

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of human beclin-1, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Human.

Cancer, Cancer Susceptibility, Cell Death, Epigenetics and Nuclear Signaling, Interspecies Interaction, Metabolism, Metabolism Processes, Microbiology, Oncoproteins/Suppressors, Pathways and Processes, Signal Transduction, Transcription, Tumor Suppressors

Plays a central role in autophagy (PubMed:23184933) . Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3- C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530) . Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521) . Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397) . May play a role in antiviral host defense.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Specific for endogenous levels of the ~60 kDa beclin1 protein phosphorylated at Ser295. Immunolabeling of the beclin-1 band is eliminated in the beclin-1 alanine substitution mutant.

Cytoplasm. Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein. Endosome membrane ; Peripheral membrane protein. Endoplasmic reticulum membrane ; Peripheral membrane protein. Mitochondrion membrane ; Peripheral membrane protein. Endosome. Cytoplasmic vesicle, autophagosome. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) .