Anti-ASRGL1 (Asparaginase Like 1) Monoclonal Antibody

ASRGL1 (Asparaginase-like protein 1), also known as CRASH, is a 308 amino acid protein belonging to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. ASRGL1 is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.

Isoaspartyl peptidase/L-asparaginase;3.4.19.5 ;3.5.1.1 ; Asparaginase-like protein 1 ; Beta-aspartyl-peptidase ; Isoaspartyl dipeptidase; L-asparagine amidohydrolase; Isoaspartyl peptidase/L-asparaginase alpha chain; Isoaspartyl peptidase/L-asparaginase beta chain; ASRGL1; ALP, CRASH

Expressed in brain, kidney, testis and tissues of the gastrointestinal tract. Present in sperm (at protein level) . Over-expressed in uterine, mammary, prostatic and ovarian carcinoma. .

Amino Acid Metabolism, Amino Acids, Metabolic Signaling Pathways, Metabolism, Neuroscience, Neurotransmitter, Pathways and Processes, Signal Transduction

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95&degC followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L- Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine. .

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.