Anti-Esrp-1 Monoclonal Antibody

Boster Bio Anti-Esrp-1 Monoclonal Antibody (Catalog # M06068-1) . Tested in WB applications. This antibody reacts with Mouse.

Product Specifications

Background

Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT) . EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Synonyms

Esrp-1, Esrp1, Rbm35a, Epithelial splicing regulatory protein 1, RNA-binding protein 35A, RNA-binding motif protein 35A

Gene Name

ESRP1

UniProt

Q3US41

Host

Mouse

Reactivity

Mouse

Immunogen

Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.

Clonality

Monoclonal

Clone

Clone: Clone: 27H12.F3.H5

Tissue Specificity

Epithelial cell-specific.

Applications

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, RNA Processing, Splicing

Purification

This product is an IgG fraction antibody purified by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with mouse Esrp-1 protein. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

MRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT) . Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Molecular Weight

75585 MW

Shipping Conditions

Due to dry ice shipping issues, this product is temporarily not available to distributors outside of the United States.

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Fragment

IgG2b

Applications Notes

This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 by western blotting in the appropriate cell lysate or extract.