Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248

This antibody reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross-reactivity with other histone proteins.

Widely expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level) . In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, expressed in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla.

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes (By similarity) . Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin (By similarity) (PubMed:16107708) .

Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin (PubMed:9634239) . In quiescent lymphocytes, associated with centromeric constitutive heterochromatin (PubMed:15564378) .