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Anti-IPO-38 HMGN1 Monoclonal Antibody

Boster Bio Anti-IPO-38 HMGN1 Monoclonal Antibody (Catalog # M03051) . Tested in IHC, WB applications. This antibody reacts with Human.

Product Specifications

Background

The specificity of mAb IPO-38 was demonstrated in different human and murine lymphoid and non-lymphoid cell lines. It does not react with mononuclear cells and granulocytes of healthy donors but does react with nuclei of blood cells of patients with different forms of leukemia and with cells from lymph nodes of patients with Hodgkin's disease and non-hodgkin's lymphoma. Also a positive reaction in nuclei of breast and colorectal tumor cells was demonstrated. IPO-38 may be a good marker of cellular proliferation during monitoring of tumor progression.

Synonyms

IPO38, HMGN1, HMG14, Non-histone chromosomal protein HMG-14, High-mobility group nucleosome-binding domain-containing protein 1

Gene Name

HMGN1

UniProt

P18608

Host

Mouse

Reactivity

Human

Immunogen

A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.

Clonality

Monoclonal

Clone

Clone: Clone: IPO-38 IgM kappa

Applications

IHC, WB

Field of Research

Domain Families, Epigenetics and Nuclear Signaling, Histones, Neurodegenerative Disease, Neurology Process, Neuroscience, Transcription

Purification

Anti-IPO-38 monoclonal antibody was purified by Protein A chromatography. This antigen was detected after 12h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross-reactivity is likely to occur with mouse and rat. Reactivity with other sources has not been determined.

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity) .

Molecular Weight

10659 MW

Shipping Conditions

Due to dry ice shipping issues, this product is temporarily not available to distributors outside of the United States.

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=M03051

Product MSDS

https://www.bosterbio.com/msds?sku=M03051

Fragment

IgM kappa

Applications Notes

Anti-IPO-38 has been tested by immunohistochemistry (frozen and formalin/paraffin) and western blot analysis on the Raji cell line and reacts with a 14 -16 kDa protein. This antibody is suitable for ELISA and immunoprecipitation. The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-100 permeabilized) . An increase of 400% is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemid) . IPO-38 does not block the binding of the Ki-67 antibody. Tonsillar tissue is typically used as a positive control.

Other Gene Names

Non-histone chromosomal protein HMG-14

Subcellular Location

Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.

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