Anti-BAG-1 Rabbit Monoclonal Antibody, Clone#RM356

Boster Bio Anti-BAG-1 Rabbit Monoclonal Antibody, Clone#RM356 (Catalog # M02423-1) . Tested in IHC, WB applications. This antibody reacts with Human.

Product Specifications

Synonyms

BAG family molecular chaperone regulator 1; BAG-1; Bcl-2-associated athanogene 1; BAG1; HAP

Gene Name

BAG1

UniProt

Q99933

Host

Rabbit

Reactivity

Human

Cross Reactivity

This antibody reacts to total human BAG-1 protein, including long (BAG-1L), medium (BAG-1M) and short (BAG-1S) isoforms

Immunogen

A peptide corresponding to human BAG-1 (Bcl-2-associated athanogene 1)

Clonality

Monoclonal

Clone

Clone: RM356

Tissue Specificity

Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level) .

Applications

IHC, WB

Field of Research

Apoptosis, Apoptotic Markers, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:27474739, PubMed:9873016, PubMed:24318877) . Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity (PubMed:12724406) . Markedly increases the anti-cell death function of BCL2 induced by various stimuli (PubMed:9305631) .