Anti-Alpha Actinin-4 ACTN4 Rabbit Monoclonal Antibody, Clone#RM287

Boster Bio Anti-Alpha Actinin-4 ACTN4 Rabbit Monoclonal Antibody, Clone#RM287 (Catalog # M01975-1) . Tested in IHC, WB applications. This antibody reacts with Human.

Product Specifications

Synonyms

Alpha-actinin-4 ; Non-muscle alpha-actinin 4 ; ACTN4

Gene Name

ACTN4

UniProt

O43707

Host

Rabbit

Reactivity

Human

Cross Reactivity

This antibody reacts to human alpha-Actinin-4.

Immunogen

A peptide corresponding to the N-terminus of human alpha-Actinin-4

Clonality

Monoclonal

Clone

Clone: RM287

Tissue Specificity

Widely expressed.

Applications

IHC, WB

Field of Research

Invasion/Microenvironment, Metabolism, Metabolism Processes, Microfilaments, Pathways and Processes, Response To Hypoxia, Signal Transduction

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable) . Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161) . Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity) . May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778) .

Molecular Weight

104854 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

Alpha-actinin-4

Subcellular Location

Nucleus. Cytoplasm. Cell junction. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC- 2, and bladder cancer KU-7) .