Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247

Boster Bio Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247 (Catalog # M01513-3) . Tested in IHC, WB applications. This antibody reacts with Human.

Product Specifications

Synonyms

Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD31; PECAM1

Gene Name

PECAM1

UniProt

P16284

Host

Rabbit

Reactivity

Human

Cross Reactivity

This antibody reacts to human CD31 (Platelet endothelial cell adhesion molecule, PECAM-1) .

Immunogen

A peptide corresponding to the cytoplasmic domain of human CD31

Clonality

Monoclonal

Clone

Clone: RM247

Tissue Specificity

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells (PubMed:18388311) . Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) (PubMed:19342684, PubMed:17580308) . Expressed on neutrophils (at protein level) (PubMed:17580308) . Isoform Long predominates in all tissues examined (PubMed:12433657) . Isoform Delta12 is detected only in trachea (PubMed:12433657) . Isoform Delta14-15 is only detected in lung (PubMed:12433657) . Isoform Delta14 is detected in all tissues examined with the strongest expression in heart (PubMed:12433657) . Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level) (PubMed:12433657, PubMed:18388311) .

Applications

IHC, WB

Field of Research

Angiogenesis, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Immunology, Signal Transduction

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308) . Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684) . Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308) . Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892) . Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892) . Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896) . Regulates bradykinin- and hyperosmotic shock- induced ERK1/2 activation in endothelial cells (PubMed:18672896) . Induces susceptibility to atherosclerosis (By similarity) .

Molecular Weight

82536 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

Platelet endothelial cell adhesion molecule

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell surface expression on neutrophils is down-regulated upon fMLP or CXCL8/IL8-mediated stimulation.