Anti-Spastin Rabbit Monoclonal Antibody, Clone#RM346

Boster Bio Anti-Spastin Rabbit Monoclonal Antibody, Clone#RM346 (Catalog # M00521) . Tested in IHC, WB applications. This antibody reacts with Human.

Product Specifications

Synonyms

Spastin ;5.6.1.1 ; Spastic paraplegia 4 protein; SPAST ; ADPSP, FSP2, KIAA1083 , SPG4

Gene Name

SPAST

UniProt

Q9UBP0

Host

Rabbit

Reactivity

Human

Cross Reactivity

This antibody reacts to human Spastin.

Immunogen

A peptide corresponding to the N-terminus of human Spastin.

Clonality

Monoclonal

Clone

Clone: RM346

Tissue Specificity

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The short isoforms may predominate in brain and spinal cord.

Applications

IHC, WB

Field of Research

ATPases, Cancer, Cytoskeleton, Cytoskeleton/ECM, Metabolism, Microtubules, Plasma Membrane, Signal Transduction

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:11809724, PubMed:15716377, PubMed:16219033, PubMed:17389232, PubMed:20530212, PubMed:22637577, PubMed:26875866) . Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866) . Severing activity is not dependent on tubulin acetylation or detyrosination (PubMed:26875866) . Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (PubMed:19000169, PubMed:21310966, PubMed:26040712) . Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex (PubMed:21310966) . Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712) . Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (PubMed:23897888) . Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (PubMed:23897888) . Probably plays a role in axon growth and the formation of axonal branches (PubMed:15716377) .

Molecular Weight

67197 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

Spastin

Subcellular Location

Membrane; Peripheral membrane protein. Endoplasmic reticulum. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Forms a intramembrane hairpin-like structure in the membrane (PubMed:20200447) . Localization to the centrosome is independent of microtubules (PubMed:15891913) . Localizes to the midbody of dividing cells, and this requires CHMP1B (PubMed:18997780) . Enriched in the distal axons and branches of postmitotic neurons (PubMed:15269182) .